TECHNOLOGY

 

Universal Kinase Recognition Through Optimized Chemistry*

KiNativ™ is based on patented biotinylated acyl phosphates of ATP or ADP that irreversibly react with protein kinases on conserved lysine residues in the ATP-binding pocket. In addition, the probes identify other ATPases such as lipid kinases and heat shock proteins, by reacting with lysine residues in their ATP-binding sites. The basic probe features are compatible with all kinases regardless of species.

Robust and Reproducible Kinase Activities

KiNativ™ broadly profiles protein kinase activities in cell lysates. The KiNativ™ probes, platform and data analysis methods provide an unprecedented ability to broadly profile protein kinase activities in cell lysates for both overall activity/expression level and sensitivity to candidate inhibitors (without the need for protein expression or substrate assay development). A high-content mass spectrometry platform, proprietary data analysis algorithms, and unique chemical probes yield robust, quantitative and reproducible results from any tissue, cell lysate, or biological fluid.

More Details

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*Patricelli, M.P., Szardenings, A.K., Liyanage, M., Nomanbhoy, T.K., Wu, M., Weissig, H., Aban, A., Chun, D., Tanner, S., Kozarich, J.W.: Functional interrogation of the kinome using acyl phosphates. Biochemistry, Jan. 16, 2007; 46(2);350